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Image Search Results
Journal: The Journal of Experimental Medicine
Article Title: Scleroderma Autoantigens Are Uniquely Fragmented by Metal-catalyzed Oxidation Reactions: Implications for Pathogenesis
doi:
Figure Lengend Snippet: Metal ions and topoisomerase I are concentrated in punctate intranucleolar structures. ( A ) HeLa cells were fixed in 1% lead acetate, and subsequently stained with 1% ammonium sulfide. Bright field microscopy demonstrates intense staining of punctate intranucleolar structures (nucleolini). ( B ) HeLa cells were stained with a monospecific human serum recognizing topoisomerase I and FITC-goat anti– human IgG, and were examined by confocal fluoresence microscopy. Diffuse nuclear staining, as well as punctate intranucleolar structures (nucleolini), are seen. Similar results were obtained with six other monospecific topoisomerase I sera. Nucleolini were never stained when similar experiments were performed using sera obtained from healthy individuals, or from Ro/La-positive lupus patients. Bar, 10 μM.
Article Snippet: Bound antibodies were visualized with
Techniques: Staining, Microscopy